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In rabbits, the highest titers of parasite growth inhibitory antibodies were obtained with the GLA-SE formulation.

In the mouse experiments, the GLA adjuvanted formulations were superior to the Nanoalum formulation with respect to antibody titer development, IFA sero-conversion rates and in vitro parasite growth-inhibitory activity. falciparum growth inhibitory activity was determined with a standardized in vitro -hypoxanthine incorporation assay. ELISA and indirect immunofluorescence assays (IFA) were used to analyse elicited IgG titers and the P. Mice and rabbits were immunized with vaccine formulations of recombinantly expressed PfCyRPA adjuvanted either with the glucopyranosyl lipid A (GLA) containing adjuvants GLA-LSQ, GLA-SE, GLA-Alum or with Nanoalum. In contrast to several previously clinically tested merozoite vaccine candidate antigens, PfCyRPA is not polymorphic, making it a promising candidate antigen for blood stage vaccine development. Plasmodium falciparum cysteine-rich protective antigen ( PfCyRPA) is an invasion complex protein essential for erythrocyte invasion.